SCREEN-WINE

Financing contract no.: 45PD/2018

Project No.: PN-III-P1-1.1-PD-2016-0518

Project implementation period: 01.05.2018-30.04.2020


Innovative strategies based on screening techniques coupled with multivariate statistical analysis used for wines authenticity assessments


Summary

Valorification of the Romanian wine-growing potential, in order to obtain sustainable competitive advantages in foreign markets, requires the development and promotion of the identity of Romanian wines, based on analytical investigations certifying quality and natural chemical composition of the wines. Consumers, regulatory bodies and winemakers are interested to have reliable and cost-effective analytical tools and information to allow the authentication of wines. The assessment of high quality wines authenticity in terms of varietal and vintage confirmation is a major concern that has gained a lot of interest internationally since these wines has always been subjected to false declaration of origin. SCREEN-WINE project aims to develop and validate new rapid alternative analytical strategies employing more accessible techniques, like screening techniques for a reliable, high-throughput and cost-effective wine varietal and vintage authentication. Thus, fingerprinting of wine organic compounds by rapid screening techniques like spectroscopic techniques (UV-Vis, FT-IR, 1H-NMR) and “electronic tongues” employing electrochemical sensors/biosensors combined with appropriate pattern recognition systems will be performed. In this respect, we consider that it is crucial to provide essential data in order to achieve several milestones including the establishment of references databases with qualitative and quantitative information of wine organic constituents, estimating the variation of spectral and analytical data observed by varietal and vintage factors, which can be compared with the variation due to the possibility to add legally up to 15% of another wine without any declaration.

Project Team

Project leader: PhD. Chem. Geană Elisabeta-Irina; https://orcid.org/0000-0003-4909-8574

National Research and Development Institute for Cryogenics and Isotopic Technologies – ICSI Rm. Valcea; Research-Development & Technological Transfer Department (RDTT) – Laboratories (http://erris.gov.ro/R&DTT-Laboratories)

Mentor: Prof. PhD. Apetrei Constantin; https://orcid.org/0000-0002-3823-4174
„Dunarea de Jos” University of Galati , European Centre of Excellence for the Environment, ECEE, Laboratory of sensors and biosensors for the analysis of food products – BIOSENS (http://erris.gov.ro/European-Centre-of-Excellenc-1):

Objectives

General objectives

The project, encouraged by the European Reference Centre for Control in the Wine Sector (ERC-CWS) will develop cost effective analytical methods that will enable an objective wines authentication, mostly, of wines varietal composition and vintage.
The SCREEN-WINE proposal is highly feasible since the technical support, together with specialized personnel and preliminary results on the research topic are already available with a proper allocation of time and financial resources. Essential data will be obtained in order to achieve specific objectives such as:

  • Investigate new alternative methodologies for classification and authentication of Romanian high quality wines, both white and red varieties;
  • Establish, implement and validate innovative and efficient analytical methods able to objectively assess the wines varietal and vintage classification;
  • To promote accessible and cost-effective strategies based on applications of screening tools of wine organic constituents (spectroscopic techniques and electrochemical “electronic tongues” devices) coupled with multivariate statistical analysis for wines classification;
  • To collect referential data and knowledge related with the optimum methods for authentication of high quality wine.
SMART objectives

The SMART indicator for analyzing the feasibility of the proposal contains the following topics:

  • Specific – determining the chemical fingerprints – spectral and electrochemical data and evaluating the biochemical properties (polyphenols, flavonoids, antioxidant activity) of authentic wines;
  • Measurable – validated screening methodologies by spectroscopic techniques – UV-Vis, FT-IR, NMR and other rapid methods like “electronic tongues” employing electrochemical devices based on different sensors designs used to determine the wines varietal composition and vintage; data bases for authenticating different red and white wine varieties and wines vintage;
  • Assignable – priority research interest at national and international level;
  • Realistic – the existing knowledge and infrastructure;
  • Time-related – coherence of the work plan; proper allocation of time and financial resources.

Expected results

  • Databases with spectral data of authentic wines which will be used for authenticity assessment of high quality wine samples.
  • Validated screening spectroscopic methodologies used to test the authenticity of high quality wines;
  • Databases with voltammetric data and electrochemical quantitative information referred to the phenolic compounds of authentic wines which will be used for authenticity assessment of unknown wine samples.
  • Validated innovative screening and quantitative electrochemical methodologies (“electronic tongue”) for wine authentication;
  • Complete alternative databases and methodologies used for wine authenticity assessment based on rapid screening methods (spectroscopic and electrochemical “electronic tongue” devices);

Budget

Total budget: 250.000 RON

Budget allocation by stages (in RON):

 

  • Stage 1: Studying the feasibility of spectroscopic techniques combined with multivariate statistical analysis (MVA) to provide rapid screening tools for wines authenticity assessment
    Total budget: 78.335
  • Stage 2: Investigation of voltammetric and amperometric “electronic tongue” (ET) sensor devices coupled with multivariate analysis as innovative screening strategies for the rapid fingerprinting and classification of authentic wines based on their tasting fingerprints
    Total budget: 140.000
  • Stage 3: Statistical management of the data
    Total budget: 31.665

Project progress: public results & dissemination

Public results

Dissemination

Manuscripts published in per review ISI and BDI journals
  • Elisabeta-Irina Geană, Victoria Artem, Constantin Apetrei, Discrimination and classification of wines based on polypyrrole modified screen-printed carbon electrodes coupled with multivariate data analysis, trimis spre publicare in revista Journal of Food Composition and Analysis, IF=2.994, status: under review
  • Elisabeta-Irina Geană, Corina Teodora Ciucure, Victoria Artem, Constantin Apetrei, Wine varietal discrimination and classification using a voltammetric sensor array based on modified screen-printed electrodes in conjunction with chemometric analysis, trimis spre publicare in revista Microchemical Journal, IF=3.206, status: under review
  • Elisabeta-Irina Geană, Corina Teodora Ciucure, Constantin Apetrei,. Electrochemical sensors coupled with multivariate statistical analysis as screening tools for wine authentication issues:  a review,  trimis spre publicare in revista Chemosensors, tracked for IMPACT FACTOR, CITESCORE 2.77 SCOPUS, status: under review
  • Elisabeta-Irina Geana, Corina Teodora Ciucure, Constantin Apetrei and Victoria Artem, Application of spectroscopic UV-Vis and FT-IR screening techniques coupled with multivariate statistical analysis for red wines authentication: varietal and vintage year discrimination, Molecules 2019, 24, 4166; doi:10.3390/molecules24224166, IF/2019: 3.06
    https://www.mdpi.com/1420-3049/24/22/4166
  • Elisabeta-Irina Geana, Corina Teodora Ciucure, Constantin Apetrei and Victoria Artem, Characterization and classification of wines based on spectrophotometric determination of wine bioactive properties, CPQ Nutrition (2019), 3(6), 1-12, Google Scholar indexed journal.
    https://www.cientperiodique.com/article/CPQNN/3/6/74
Scientific manifestations:
  • 8th Edition of Scientific Conference of Doctoral Schools, SCDS-UDJG, Perspectives and challenges in doctoral research, 18-19 iunie 2020, Universitatea “Dunarea de Jos”, Galati,  : http://www.cssd-udjg.ugal.ro/: Elisabeta-Irina Geană, Victoria Artem, Constantin Apetrei, Discrimination and classification of red wines based on FTIR data coupled with multivariate data analysis, prezentare orala
  • 8th Edition of Scientific Conference of Doctoral Schools, SCDS-UDJG, Perspectives and challenges in doctoral research, 18-19 iunie 2020, Universitatea “Dunarea de Jos”, Galati,  : http://www.cssd-udjg.ugal.ro/: Elisabeta-Irina Geană, Corina Teodora Ciucure,Victoria Artem, Constantin Apetrei, Discrimination of white wines using UV-Vis and FT-IR fingerprinting technologies combined with chemometrics, prezentare orala
  • 7th Edition of Scientific Conference of Doctoral Schools, SCDS-UDJG, Perspectives and challenges in doctoral research, 13-14 iunie 2019, Universitatea “Dunarea de Jos”, Galati, : http://www.cssd-udjg.ugal.ro/
    Elisabeta-Irina Geană*, Constantin Apetrei, Voltammetric sensors in the analysis of wine redox-active compounds – prezentare orala: link
  • 9th International Symposium EuroAliment “Inovative minds for future food”, 05-06 septembrie 2019, Galati, Romania: http://www.euroaliment.ugal.ro/euro-aliment_2019.htm
    Elisabeta-Irina Geană, Corina Teodora Ciucure, Victoria Artem, Constantin Apetrei, Exploring the capabilities of polypyrrole modified electrochemical sensors in combination with multivariate statistical analysis for wine authentication purpose – prezentare orala: link
  • 12th International Conference „Processes In Isotopes And Molecules”, 25-27 Septembrie 2019, Cluj Napoca, Romania: http://pim.itim-cj.ro/index.html
    Elisabeta-Irina Geană, Victoria Artem, Constantin Apetrei, Electrochemical biosensing of phenolic compounds from wines -prezentare poster: link
  • 18th International Conference “Life sciences for sustainable development”, 26-28.09.2019, Cluj Napoca, Romania: http://symposium.usamvcluj.ro/
    Elisabeta-Irina Geana, Corina Teodora Ciucure, Constantin Apetrei, Victoria Artem, Addressing wine authenticity using biochemical properties and chemometrics, prezentare poster: link
  • 2nd Food Chemistry Conference, 17-19 Septembrie 2019, Sevilia, Spania: https://www.elsevier.com/events/conferences/food-chemistry-conference
    Elisabeta-Irina Geană, Corina Teodora Ciucure, Victoria Artem, Constantin Apetrei, UHPLC-MS/MS fingerprinting of wine characteristic compounds profiles combined with multivariate analysis for wine authentication approaches – prezentare poster: link
  • 4th International Conference on Analytical Chemistry: Analytical Chemistry for a Better Life, RO-ICAC’2018, 1-3 September 2018, Bucharest, Romania: https://roicac2018.wordpress.com/

Elisabeta-Irina Geană, Constantin Apetrei*, UV-Vis spectroscopy used for classification of Romanian wines, poster: link

  • 22nd Conference “New Cryogenic and Isotope Technologies for Energy and Environment” – EnergEn 2018, 24 – 26 October 2018, Băile Govora, Romania: https://www.icsi.ro/conference/

Elisabeta-Irina Geană, Corina Teodora Ciucure, Raluca Popescu, Constantin Apetrei2*, Victoria Artem, Application of spectroscopic techniques coupled with multivariate statistical analysis for wines authenticity assessment, poster: link

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